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Leverandør: G-Biosciences
Beskrivelse: TCM-ProteaseArrest™ is a mixture of protease inhibitors with a broad specificity for the inhibition of serine, cysteine, aspartic (acid) proteases and aminopeptidases.

Leverandør: G-Biosciences
Beskrivelse: RUBEO™ is a fluorescent protein stain that is ideal for staining proteins in 1D and 2D electrophoresis gels.

Leverandør: G-Biosciences
Beskrivelse: Immobilised β-cyclodextrin is designed to allow refolding of recombinant proteins that were aggregated or formed inclusion bodies when expressed in the genetically engineered cells. The refolding of misfolded proteins is adapted from GroE chaperone refolding mechanism.

Leverandør: G-Biosciences
Beskrivelse: The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP.

Varenummer: (GENO786-1999)
Leverandør: G-Biosciences
Beskrivelse: Bsu DNA Polymerase. Large fragment is from Bacillus subtilis and is modified to retain its 5'→ 3' DNA polymerase activity with its 5'→ 3' exonuclease domain removed. It also lacks the 3'→ 5' exonuclease activity.
UOM: 1 * 1 Each


Leverandør: G-Biosciences
Beskrivelse: T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes. The protein forms a right-handed nucleoprotein complex on single ssDNA called the presynaptic filament that can search for homology in duplex DNA and pair the recombining DNA molecules to form a DNA joint molecule. This process is aided by ssDNA binding proteins and recombination mediators. The filament is then elongated by DNA polymerase with the newly synthesised strand displacing the old strand. The newly synthesised duplex DNA can then act as a template for the next cycle to achieve exponential amplification of dsDNA. <i>E.coli</i> cells expressing recombinant T4 UvsX gene.

Varenummer: (GENO786-1992)
Leverandør: G-Biosciences
Beskrivelse: T4 recombination mediator protein, UvsY plays important roles in homologous recombination (HR). During HR, the UvsX recombinase competes with the prebound single-stranded binding protein gp32 for DNA-binding sites and UvsY stimulates this filament nucleation event.
UOM: 1 * 1 Each


Varenummer: (BKC-15)
Leverandør: G-Biosciences
Beskrivelse: G-Biosciences' Sodium meta -Periodate is a mild oxidizing agent that converts carbohydrates to activated active aldehydes
UOM: 1 * 5 g


Varenummer: (786-210)
Leverandør: G-Biosciences
Beskrivelse: CytoScan™ LDH Cytotoxicity assay quantitatively measures the cytosolic, lactate dehydrogenase (LDH) enzyme, which is released from damaged cells. The released LDH is measured with a coupled enzymatic reaction that results in the conversion of a tetrazolium salt (iodonitrotetrazolium; (INT)) into a red color formazan by diaphorase. The resulting formazan is measured at 490 nm.
UOM: 1 * 1 Each

Certifikater


Varenummer: (786-232)
Leverandør: G-Biosciences
Beskrivelse: The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.
UOM: 1 * 1 Each


Varenummer: (786-246)
Leverandør: G-Biosciences
Beskrivelse: Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.
UOM: 1 * 1 Each


Leverandør: G-Biosciences
Beskrivelse: For the rapid purification of soluble, 6X His or GST tagged proteins.

Leverandør: G-Biosciences
Beskrivelse: Natriumhydrogenkarbonat

Leverandør: G-Biosciences
Beskrivelse: L (+)-Ascorbinsyre

Leverandør: G-Biosciences
Beskrivelse: These disposable spin columns are designed for protein purification. These spin columns are uniquely designed, with a snap off end converting to a closure for the column for easy manipulation and use.

Leverandør: G-Biosciences
Beskrivelse: <i>Pfu</i> DNA Polymerase, derived from the hyperthermophilic archae <i>Pyrococcus furiosus</i>, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). <i>Pfu</i> DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that<i> Pfu</i> DNA Polymerase-generated PCR fragments will have fewer errors than <i>Taq</i>-generated PCR inserts. Using <i>Pfu</i> DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. <i>Pfu</i> DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

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