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Product Image-733-0950

ABsolute Blue™ qPCR Mixes

Leverandør: Thermo Fisher Scientific
Beskrivelse: ABsolute Blue™ mastermix inkorporerer en inert blå farve med henblik på at øge kontrasten mellem reagens og plast betydeligt, hvilket gør verifikation af MasterMix-dispensering hurtig, nem og idiotsikker. Anvendelsesområder omfatter genekspression, siRNA-validering, SNP-bestemmelse og gentypebestemmelse.

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Maxima™ SYBR green/fluorescein QPCR master mix (2X)

Leverandør: Thermo Fisher Scientific
Beskrivelse: The Maxima™ SYBR® Green/Fluorescein qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. It contains SYBR® Green I dye and is supplemented with fluorescein passive reference dye. Fluorescein is included into the master mix to serve as an internal reference for normalisation
of the SYBR Green I fluorescent signal between different wells to compensate for any instrument or pipetting variation. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG)*. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. The use of Maxima™ SYBR® Green/Fluorescein qPCR Master Mix in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

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EvaGreen® Plus dye, 20X in Water

Leverandør: Biotium
Beskrivelse: EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

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ABsolute™ qPCR Low ROX Mixes

Leverandør: Thermo Fisher Scientific
Beskrivelse: ABsolute™ qPCR Low ROX Mixes er optimeret til anvendelse sammen med realtidsinstrumenter, der er i stand til med større følsomhed at påvise farver med lang bølgelængde, for eksempel ABI 7500. Det lavere niveau af ROX fremmer normalisering hen over pladen og bevarer samtidig følsomheden i analysen. Blandingerne indeholder alle de nødvendige komponenter til at udføre kvantitativ PCR® med undtagelse af skabelon og primere/prober. 2x qPCR mix indeholder en farmaceutisk reaktionsbuffer, der sørger for en høj følsomhed, specificitet og reproducerbarhed.

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Maxima™ H minus cDNA synthesis master mix

Leverandør: Thermo Fisher Scientific
Beskrivelse: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimised for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.

Certifikater

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SPINeasy® RNA Kit for Feces

Varenummer: (116556050.)
Leverandør: MP Biomedicals
Beskrivelse: <p>The SPINeasy® RNA Kit for Feces delivers high yields of pure RNA without detectable DNA contamination in less than 20 min, making it particularly suitable for downstream applications, such as qPCR and RT-qPCR without the need of further inhibitor/DNA removal steps.</p>
UOM: 1 * 1 Each

New Product


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NGS FFPE QC kit

Leverandør: AGILENT
Beskrivelse: The Agilent NGS FFPE QC kit is a qPCR-based assay that enables functional DNA quality assessment of input DNA prior to preparation of next generation sequencing (NGS) libraries.

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RNA isolation, total RNA kit, E-Z 96®

Leverandør: OMEGA BIO-TEK
Beskrivelse: The E-Z 96® Total RNA Kit is designed for isolation of total cellular RNA from up to 5×10⁶ cultured cells or soft tissues. This kit can process single or multiple samples in less than 60 minutes. Utilising HiBind® silica plate technology, the need for phenol/chloroform extractions, CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl are eliminated. Samples are lysed in a denaturing lysis buffer which inactivates RNases. Binding conditions are adjusted and the lysate is transferred to a 96-well HiBind® RNA Plate where the RNA is purified via three wash steps. High quality RNA is eluted in RNase-free water. RNA purified using the E-Z 96® Total RNA method is ready for applications such as RT-PCR, qPCR, differential display, microarrays, and other downstream applications.

MSDS

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Maxima™ H minus cDNA synthesis master mix, with dsDNase

Leverandør: Thermo Fisher Scientific
Beskrivelse: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.

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Cell lysis analysis kit, SideStep II

Varenummer: (AGLS400900)
Leverandør: AGILENT
Beskrivelse: Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.
UOM: 1 * 1 Items

Certifikater


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Mimix™ Pan-Cancer 6-Fusion Panel (Negative Control)

Varenummer: (DHARHD835)
Leverandør: Horizon Discovery
Beskrivelse: Pan-cancer 6-fusion panel (negative control) FFPE RNA reference standard is a highly-characterised, biologically-relevant quality control material used to assess the performance of targeted NGS RNA-seq, endpoint reverse-transcription PCR (RT-PCR), RT-qPCR and RT droplet digital PCR (RT-ddPCR) assays aimed at detecting gene fusions.
UOM: 1 * 1 Items

New Product

Market Source Item This is a MarketSource item. Additional charges may apply

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VWR®, Real-Time PCR Plates, 96-Well and 384-Well

Leverandør: VWR Collection
Beskrivelse: VWR® 96- and 384-well Real-Time PCR plates are compatible with most leading Real-Time PCR instruments including most BioRad, Applied Biosystems, Roche Lightcycler and Analytik Jena models.

Environmentally Preferable

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SPINeasy® RNA Extraction Kit for Virus, MP Biomedicals

Varenummer: (116537050.)
Leverandør: MP Biomedicals
Beskrivelse: SPINeasy Virus RNA kit is a silica-membrane spin-column kit that enables quick and convenient extraction of virus RNA from cell culture media and bodily fluids such as saliva and serum, without the use of toxic substances such as phenol and chloroform. Through a simple workflow, virus RNA is typically extracted within 20 minutes and is immediately available for downstream applications such as RT-PCR and RT-qPCR.
UOM: 1 * 1 Each
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J.T.Baker®, PCR Tubes and Caps for qPCR, 8-Strips

Leverandør: Avantor
Beskrivelse: PCR 8-tube strips and 8-cap strips are made of high-quality PP resin. Clear caps are compatible with qPCR application.

Environmentally Preferable

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VWR®, Sealing films, Non Tacky, for qPCR, Storage and Crystallisation

Leverandør: VWR Collection
Beskrivelse: 50 µm polyolefin films with 50 µm inert encapsulated silicone adhesive. Especially suited for qPCR, storage and protein crystallisation applications. The encapsulated silicone adhesive is non tacky until pressed against the sealing surface, at which time adhesive is released only in sealing areas to form the strongest available heat resistant seal around each well on the plate.

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Whole Blood RNA Purification Mini kit, GeneJET™

Varenummer: (K0761)
Leverandør: Thermo Fisher Scientific
Beskrivelse: GeneJET™ Whole Blood RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from whole blood and related body fluids. The kit utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 15 minutes. The purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analysis.
UOM: 1 * 1 Each
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