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Leverandør: Biowest
Beskrivelse: Antibiotic-antimycotic used in tissue culture to prevent growth of bacteria (gram positive and gram negative), yeast and fungi. Penicillin acts by inhibiting bacterial cell wall synthesis.
Varenummer: (L0950-100)
Leverandør: Biowest
Beskrivelse: Accustase is a ready to use cell detachment solution of proteolytic and collagenolytic enzymes. Accutase is a direct gentle replacement for trypsin solution. It can also be used on suspension cells to reduce their agglutination in order to ease counting.
UOM: 1 * 100 mL

Varenummer: (L0207-500)
Leverandør: Biowest
Beskrivelse: Schneider’s Insect Medium was developed to support the growth of excised imaginal discs from the fruit fly, <i>Drosophila melanogaster</i>. When supplemented with 5 to 20% heat-inactivated foetal bovine serum Schneider's mediuam has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.
UOM: 1 * 500 mL

Leverandør: Biowest
Beskrivelse: Colcemid arrests the division of cells in mitosis. It prevents the formation of the spindle apparatus responsible for cell division, thereby permitting an accumulation of metaphases.
Leverandør: Biowest
Beskrivelse: Amphotericin B used in tissue culture to prevent growth of yeasts and fungi. It changes the function and integrity of eucaryotic cell membranes by forming complexes with sterols (cholesterol) hence having no effect on bacteria. Cause leakage of glucose.
Varenummer: (X0550-100)
Leverandør: Biowest
Beskrivelse: L(+)-Glutamin 0.2 mol/l i vandigopløsning
UOM: 1 * 100 mL

Leverandør: Biowest
Beskrivelse: RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukaemia cells.
Leverandør: Biowest
Beskrivelse: Studies to determine the nutritional requirements of many cells have been in progress since Eagle's first reports. The major essential nutrients were identified, and work became focussed on the media requirements of individual cell types. Many media designed for these purposes are now available. Among the first of these media, developed initially to study hormonal requirements of cells in culture, was a mixture of DMEM and Ham's F12 medium, known as DMEM-Ham's F12.
Leverandør: Biowest
Beskrivelse: Antibiotic G418 is an aminoglycoside antibiotic similar in structure to gentamycin. It exhibits toxicity towards both eukaryotic and prokaryotic cells. The optimal concentration for selection and maintenance must be determined for each cell line.
Leverandør: Biowest
Beskrivelse: Ham's F10 medium is intended to support the growth of Chinese Hamster Ovary cells under serum-free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cell lines.
Leverandør: Biowest
Beskrivelse: Gentamicin sulfate acts against gram-negative, gram-positive bacteria and mycoplasma.
Leverandør: Biowest
Beskrivelse: The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.
Leverandør: Biowest
Beskrivelse: Sterile filtered foetal bovine serum (FBS) derived from clotted whole blood aseptically collected from foetus via cardiac puncture. Each manufactured batch is rigorously controlled, from the collection of serum through to final packaging.

Leverandør: Biowest
Beskrivelse: Bovine serum albumin (BSA) is commonly used in cell culture protocols, particularly where protein supplementation is necessary and the other components of serum are unwanted. In cell culture, its main role is as a carrier of small molecules. Because of its negative charge, BSA binds water, salts, fatty acids, vitamins and hormones, then carries these bound components between tissues and cells. The binding capacity also makes BSA an effective scavenger to remove toxic substances, including pyrogens, from the medium.
Leverandør: Biowest
Beskrivelse: The long term cultivation of cells requires the addition of serum. When properly supplemented, Medium 199 has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and<i> in vitro</i> cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.
Leverandør: Biowest
Beskrivelse: Rat serum is the liquid fraction of whole blood that is collected after the blood is allowed to clot; it is the blood plasma with the fibrinogens removed. Serum includes all proteins not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances. Rat serum is used as a blocking agent in immunoassays and controls.

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