Du Søgte efter: Proteinassays

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.


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Leverandør: G-Biosciences
Beskrivelse: CB-X™ Protein Assay is a Coomassie dye based protein assay based on the Bradford Protein Assay. For protein samples in simple, uncomplicated aqueous buffers CB-X™ is a highly sensitive, single reagent assay that can be performed in 5 minutes. For complicated protein samples, CB-X™ Protein Assay is supplied with reagents to clean up the samples and remove all reagents and chemicals that interfere with accurate protein estimation in a single step. These reagents include detergents, chaotropes, reducing agents, alkylating agents, sugars, high salt concentrations, buffering agents and chelating agents. The clean up stage and subsequent protein assay is performed in a single tube to ensure no protein loss and to maintain the accuracy of the assay.

Varenummer: (USBIO8061-100)
Leverandør: US Biological
Beskrivelse: Osteocrin is a recently identified secreted protein expression of which was only detected in bone, peaking just after birth and decreasing markedly with age. A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11,4 kD. In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures.
UOM: 1 * 1 Each


Leverandør: G-Biosciences
Beskrivelse: CB™ Protein Assay, a Coomassie dye based protein assay based on the Bradford Protein Assay, is suitable for the simple and rapid estimation of protein concentration. The binding of protein to the dye results in a colour change from brown to blue. The change in colour density is proportional to protein concentration. Protein estimation can be performed using as little as 0,5 µg protein.

Varenummer: (PIER23200)
Leverandør: Thermo Fisher Scientific
Beskrivelse: The Pierce™ Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595 nm) total protein concentration compared to a protein standard.
UOM: 1 * 1 Each

Varenummer: (AATB11111)
Leverandør: AAT BIOQUEST
Beskrivelse: Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.
UOM: 1 * 1 Each


Leverandør: Thermo Fisher Scientific
Beskrivelse: This BCA Protein Assay Kit - Reducing Agent Compatible enables the measurement of protein concentration in samples that contain thiol-reductants dithiothreitol (DTT) and 2-mercaptoethanol (BME).

Varenummer: (AATB22332)
Leverandør: AAT BIOQUEST
Beskrivelse: REASH is a resorufin derivative, modified to contain two arsenic atoms at a set distance from each other.
UOM: 1 * 1 Each


Leverandør: Thermo Fisher Scientific
Beskrivelse: Pierce™ Protease Assay Kits are an ideal choice for performing routine assays necessary during the isolation of proteases, or for identifying the presence of contaminating proteases in protein samples. These protease assays are also ideal for studying pH or temperature vs. activity profiles of purified proteases.
Varenummer: (PIER21115)
Leverandør: Thermo Fisher Scientific
Beskrivelse: Pierce™ biotinylated protein interaction pull-down kit contains the necessary components to capture and purify interactors of a biotin-labeled protein or ligand.
UOM: 1 * 1 Each


Leverandør: Thermo Fisher Scientific
Beskrivelse: Micro BCA™ protein assay kit is a specialised version of Pierce™ BCA protein assay for determining the protein concentration of dilute samples.

Varenummer: (PIER48895)
Leverandør: Thermo Fisher Scientific
Beskrivelse: Marfey's Reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide) reacts with primary amines to enable the quick and easy separation and quantitation of optical isomers of amino acids by reverse-phase chromatography.
UOM: 1 * 50 mg


Varenummer: (PIER23215)
Leverandør: Thermo Fisher Scientific
Beskrivelse: The Compat-Able™ Protein assay preparation reagent set eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.
UOM: 1 * 500 mL


Varenummer: (PIER23239)
Leverandør: Thermo Fisher Scientific
Beskrivelse: The Thermo Scientific™ Compat-Able™ Coomassie Plus Protein Assay Kit eliminates interfering substances from protein assay samples to make them compatible with BCA and Bradford assays.
UOM: 1 * 1 Each


Varenummer: (71840-3)
Leverandør: Merck Millipore (Novagen)
Beskrivelse: The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.
UOM: 1 * 125 µl


Leverandør: Thermo Fisher Scientific
Beskrivelse: The Pierce™ Firefly Luciferase Flash Assay Kit provides reagents to measure red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.

Leverandør: Thermo Fisher Scientific
Beskrivelse: This antibody labeling system uses nickel chelated agarose to temporarily immobilise antibody molecules via their histidine-rich Fc regions. Once held in place on the gel, the antibody can be biotinylated at either sulfhydryl groups (after mild reduction or disulfide bonds) or primary amines. Excess labeling reagent and by-products are then washed away before recovering the labeled and purified antibody from the gel using a mild imidazole solution. No gel filtration or dialysis are needed.

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