Du Søgte efter: Proteinassays

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.

Accurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. When it is necessary to determine the total protein concentration in a sample, one of the first factors to consider is the selection of a protein assay method. These choices are typically based upon the compatibility of the method with the samples to be assayed. Each method has its own advantages and disadvantages.


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Varenummer: (786-20LATEX)
Leverandør: G-Biosciences
Beskrivelse: A simple and reliable method for estimation of protein contamination in latex. Detects as low as 25 to 40 μg protein/gm latex (or 25 to 50 ppm). Protein solutions applied to the test strips form compact and symmetrical spots, their diameter being directly proportional to protein concentration. Protn-Latex™ latex protein extraction buffer extracts protein from the latex sample, which is then applied to the test strips and rapidly developed. By measuring the Ø of protein spots with the Protn-Latex™ card, the range of protein concentration in the sample can be determined.
UOM: 1 * 1 Each


Varenummer: (488250-1)
Leverandør: Merck Millipore (Calbiochem‎)
Beskrivelse: Non-Interfering Protein Assay™ Kit is an easy-to-use protein assay that overcomes interference of agents found in protein solutions including detergents, chelating agents, reducing agents, amines, sugars, urea, and others. The Universal Protein Precipitating Agent (UPPA™ Reagent) is used to precipitate and immobilize the protein in the tube while the interfering reagents are removed.
UOM: 1 * 1 Each


Varenummer: (PIER23275)
Leverandør: Thermo Fisher Scientific
Beskrivelse: Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay is an easy-to-use colorimetric microplate assay designed specifically to improve the sensitivity and reproducibility of quantitation of peptide mixtures.
UOM: 1 * 1 Each


Varenummer: (PIER21140)
Leverandør: Thermo Fisher Scientific
Beskrivelse: Pierce™ Streptavidin Poly-HRP conjugate is biotin-binding protein conjugated with polymers of horseradish peroxidase, enabling signal amplification and detection of biotinylated antibodies for IHC and other methods.
UOM: 1 * 0,5 mL

MSDS


Varenummer: (BIOVK251-100)
Leverandør: Abcam
Beskrivelse: Simple assay to detect total glutathione.
UOM: 1 * 1 Each


Leverandør: Abnova
Beskrivelse: Biotin Labelling Kit is used for simple and rapid preparation of biotin-labeled antibodies and proteins.

Varenummer: (80-6483-56)
Leverandør: Cytiva
Beskrivelse: 2-D Quant Kit is designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques such as 2-D electrophoresis, SDS-PAGE, or IEF.
UOM: 1 * 1 Each

Certifikater


Leverandør: MP Biomedicals
Beskrivelse: Histones are soluble in water (10 mg/ml) or 0,5 N HCl (10 mg/ml), yielding a clear to hazy solution depending on the fraction. They are soluble in 6 M urea (4 to 10 mg/ml), but this will denature the histones.

Varenummer: (733-1879)
Leverandør: Vector Laboratories
Beskrivelse: ChromaLink™ Biotin contains a UV-traceable chromophore to enable reproducibility labelling. The degree of biotinylation can be measured in minutes, without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple and direct UV scan, biotin incorporation can be quantified and reproducible production of consistent batches ensured. ChromaLink™ labelling can be paired with NanoLink™ Streptavidin Magnetic Beads for many types of assay development.
UOM: 1 * 1 Each


Varenummer: (VECTS-9011-1)
Leverandør: Vector Laboratories
Beskrivelse: The Protein-Oligo Conjugation kit is designed to easily and efficiently conjugate two separate protein-oligo conjugates. This kit is flexible so that researchers with little or no conjugation experience can make their own custom protein-oligo conjugates to suit their needs. It includes all of the necessary components - including S-HyNic and S-4FB - and protocols for easy and specific crosslinking of any protein with any oligo up to 100 base pairs in length.
UOM: 1 * 1 Each


Varenummer: (UPTIUP36859A)
Leverandør: Uptima
Beskrivelse: High quality, optimised reagents for the determination of protein concentration by the bicinchoninic acid method.
UOM: 1 * 1 Items

MSDS


Leverandør: Uptima
Beskrivelse: The BCA method is very sensitive for protein detection. Peptide bonds and the four amino acids cysteine, cystine , tryptophan and tyrosine are relevant to the determination. The formation of Cu (I) ions by proteins under alkaline conditions corresponds to the biuret method of determination. A Cu (I ) ion is then bound by two molecules of BCA to a red colour complex. This complex can be detected very sensitively.

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