Du Søgte efter: Merck Millipore (Novagen)

Leverandør: Merck Millipore (Novagen)

Beskrivelse: NovaBlue competent cells are designed for convenient and reliable plasmid transformation. NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids), and <i>recA</i> and <i>endA</i> mutations, which result in high yields of excellent-quality plasmid DNA. Standard NovaBlue and convenient NovaBlue Singles™ are ideal for routine cloning, while NovaBlue GigaSingles™ are most suitable for applications requiring higher transformation efficiencies.

Varenummer: (71546-3)

Leverandør: Merck Millipore (Novagen)

Beskrivelse: The Novagen® BacMagic™-1 transfection kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

UOM: 1 * 1 Each

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Varenummer: (70976-3)

Leverandør: Merck Millipore (Novagen)

Beskrivelse: The SpinPrep™ PCR Clean Up Kit is designed for the rapid purification of DNA amplified by PCR. The 10-minute procedure involves addition of a binding buffer followed by adsorption of the DNA to a silica membrane in a spin column format. Following a wash step, the DNA is eluted in low salt buffer. This kit removes DNA polymerises, dNTPs, salts, and >99% of primers so that they do not interfere with downstream applications such as cloning, sequencing, or labelling. PCR products from 100 to >12000 bp can be cleaned up, with recoveries of amplified DNA in the range of 60 to 90% under standard conditions.

UOM: 1 * 1 Each

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Varenummer: (71285-3)

Leverandør: Merck Millipore (Novagen)

Beskrivelse: BCA Protein Assay is a simple and reliable protein quantification method. It can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration.

UOM: 1 * 1 Each

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Varenummer: (72181-3)

Leverandør: Merck Millipore (Novagen)

Beskrivelse: 293-Free™ transfection reagent has a unique polycationic liposomal formulation and is designed expressly for transfection of HEK 293 cells grown in suspension culture. It is ideal for mammalian protein production. 293-Free™ transfection reagent is provided as a sterile, ready to use solution. The 1 ml size provides enough reagent to transfect a litre of culture.

UOM: 1 * 1 mL

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Leverandør: Merck Millipore (Novagen)

Beskrivelse: Pellet Paint® Co-Precipitant is a visible dye-labelled carrier formulated specifically for use in alcohol precipitation of nucleic acids. The 2-minute precipitation does not require low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even the most dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink colour. The pellet can be easily followed during washing steps and this prevents losses during handling and ensures thorough pellet re-suspension.

Leverandør: Merck Millipore (Novagen)

Beskrivelse: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Varenummer: (69022-3)

Leverandør: Merck Millipore (Novagen)

Beskrivelse: The thrombin cleavage capture kit is designed for cleavage of fusion proteins followed by convenient and quantitative removal of thrombin protease. The method is based on the use of biotinylated thrombin for digestion and its subsequent removal with streptavidin agarose.

UOM: 1 * 1 Each

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Varenummer: (72157-3)

Leverandør: Merck Millipore (Novagen)

Beskrivelse: The BacMagic™-2 DNA Kit provides a positive selection for creating baculoviruses recombinants in preparation for generating recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification.

UOM: 1 * 1 Each

Tilbud

Leverandør: Merck Millipore (Novagen)

Beskrivelse: This His Tag® monoclonal antibody is a mouse monoclonal antibody (IgG1) directed against the His tag sequence encoded by many of the expression vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context.

Leverandør: Merck Millipore (Novagen)

Beskrivelse: ColiRollers™ plating beads are a specially treated glass bead which offers a better way to perform plating bacteria for transformation and molecular cloning applications. ColiRollers™ beads eliminate the inconsistencies of conventional 'hockey stick' methods.

MSDS

Leverandør: Merck Millipore (Novagen)

Beskrivelse: BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Varenummer: (69182-3)

Leverandør: Merck Millipore (Novagen)

Beskrivelse: Desoxyribonuclease I

UOM: 1 * 1.000 EE

Tilbud

Varenummer: (69067-3)

Leverandør: Merck Millipore (Novagen)

Beskrivelse: The Enterokinase Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins followed by rapid, affinity-based capture and removal of enterokinase. Following cleavage of the target protein, rEK is removed with >99% efficiency from the reaction by affinity capture on EKapture™ Agarose. Following capture of rEK, the EKapture Agarose is removed by spin filtration, because the same buffer conditions are used for both cleavage and capture no buffer changes are necessary.

UOM: 1 * 1 Each

Tilbud

Varenummer: (71759-4)

Leverandør: Merck Millipore (Novagen)

Beskrivelse: Overnight Express™ Autoinduction NMR Medium, 5 L kit

UOM: 1 * 1 Each

Tilbud

Varenummer: (69278-3)

Leverandør: Merck Millipore (Novagen)

Beskrivelse: PCR Markers indeholder en blanding af otte definerede DNA-fragmenter i området fra 50 til 2000 bp ved praktiske størrelsesintervaller og bruges til karakterisering af små DNA-produkter. Markørerne leveres i en gel loading buffer, som indeholder to sporfarver som ikke påvirker UV-belysningen af ethidiumbromid-farvede bånd. Et separat rør med 6X Loading buffer er inkluderet. Hvert rør med markører rækker til 50 baner på TBE- eller TAE-agarose farvet med ethidiumbromid. Den anbefalede mængde til loading (5 µl) producerer bånd af ensartet intensitet som er klare, skarpe og lette at fotografere.

UOM: 1 * 1 Items

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