The Melon Gel IgG purification kit offers a simpler and faster alternative to traditional Protein A and Protein G methods for purification of human, mouse and rabbit IgG from serum and other sources.
- IgG purification with Melon gel resin does not involve binding, washing, and multiple elution steps required by Protein A and G
- Antibody purification from serum is four to six times faster than Protein A or G methods
- For many species of serum antibody recovery is greater than 90% with purity greater than 80%
- Reduced need to concentrate antibody before storage or use
- Works with a wide range of antibodies including many that do not purify well on Protein A or G
- No harsh antibody elution conditions helps retain IgG activity
- Melon gel resin can be used for multiple antibody purifications
Enable purification of antibodies from serum and other sample types. Melon gel resin binds and removes serum proteins, thereby allowing pure IgG to be collected in the flow-through fraction.
Melon gel resin contains a proprietary ligand that retains most protein found in serum, ascites and culture supernatants, while allowing IgG to pass through the support and be collected in the flow-through fraction. Because this gel is not a bind-and-release support, it is extremely fast and gentle to ant ibodies, resulting in antibody preparations of high purity and high activity.
Furthermore, antibodies purified with Melon gel resin are recovered ready for use in immunoassays or subsequent conjugation or modification steps; there is no need to neutralise or desalt the recovered antibody to remove harsh elution buffers.
Store at 4 °C.
Leveringsinformation: Includes Melon gel support (25 ml), Melon gel purification buffer (dry mix to make 100X) (1 L) and Melon gel regenerant (dry mix to make 1X) (1 L).
