Du Søgte efter: Kloningssæt og ligering

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signalling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signalling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Leverandør: AGILENT
Beskrivelse: The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cl...

Varenummer: (AGLS600191)
Leverandør: AGILENT
Beskrivelse: Pfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosus hyperthermophilic marine archaebacterium that catalyses linkage ...
UOM: 1 * 1 Items


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Leverandør: AGILENT
Beskrivelse: The StrataClone PCR cloning kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activi...

Leverandør: AGILENT
Beskrivelse: For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency a...

Leverandør: Thermo Fisher Scientific
Beskrivelse: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme ...

Varenummer: (EL0016)
Leverandør: Thermo Fisher Scientific
Beskrivelse: This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The ...
UOM: 1 * 1 Each

Certifikater


Leverandør: AGILENT
Beskrivelse: The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve...

Leverandør: SGI - DNA Synthetic Genomics
Beskrivelse: Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location ...

Leverandør: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire gen...
Varenummer: (GA2100-S)
Leverandør: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® Method is a well-established assembly reaction that can be leveraged to join multiple, mutagenised DNA fragments with overlapping...
UOM: 1 * 1 Each

MSDS


Leverandør: Thermo Fisher Scientific
Beskrivelse: The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expressio...

Leverandør: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultr...
Leverandør: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as enti...
Leverandør: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® Hi-Fi 1-Step Master Mix (2X) contains a proprietary mixture of enzymes and reagents optimised to facilitate one-step assembly of ...
Leverandør: Merck Millipore (Novagen)
Beskrivelse: Novagen® T7Select® Phage Display System takes advantage of the properties of bacteriophage T7. This system is easy to use and has the capacity to disp...

Leverandør: Merck Millipore (Novagen)
Beskrivelse: Clonables™ 2X ligation premix is a single solution containing optimised concentrations of T4 DNA ligase, buffer, stabiliser, and cofactors needed for ...

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