Du Søgte efter: Ekstraktions buffere

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.

Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.


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Leverandør: Avantor
Beskrivelse: J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

Environmentally Preferable

Varenummer: (M334-100ML)
Leverandør: VWR Chemicals
Beskrivelse: Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.
UOM: 1 * 100 mL


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Varenummer: (TNB-4300-L100)
Leverandør: Tonbo Biosciences
Beskrivelse: RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent.
UOM: 1 * 100 mL

Certifikater


Varenummer: (ENZOADI801496)
Leverandør: ENZO LIFE SCIENCES
Beskrivelse: The RIPA liquid cell lysis buffer composes of 50 mM TRIS-hydrogen chloride, pH 7,4, containing 150 mM sodium chloride, 1 mM EDTA, 1 mM EGTA, 1,2% Triton X-100, 0,5% sodium deoxycholate, and 0,1% SDS.
UOM: 1 * 100 mL

New Product


Leverandør: G-Biosciences
Beskrivelse: Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including <i>E.coli</i> strains.

Leverandør: OMEGA BIO-TEK
Beskrivelse: The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.
Varenummer: (ENZOADI801339)
Leverandør: ENZO LIFE SCIENCES
Beskrivelse: Cell lysis buffer 4, Enzo Life Sciences
UOM: 1 * 100 mL

New Product


Varenummer: (AGLS400900)
Leverandør: AGILENT
Beskrivelse: Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.
UOM: 1 * 1 Items

Certifikater


Leverandør: Avantor
Beskrivelse: J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.
Varenummer: (TCIAB2904-100ML)
Leverandør: TCI
Beskrivelse: Guanidine Hydrochloride Buffer Solution, 6 mol/L, pH-værdi: 8,7, TCI
UOM: 1 * 100 mL


Leverandør: ENZO LIFE SCIENCES
Beskrivelse: Concentrated lysis buffer for preparation of cell and tissue extracts.

New Product

Varenummer: (ENZOBMLKI1170030)
Leverandør: ENZO LIFE SCIENCES
Beskrivelse: Caspase cell lysis buffer, Enzo Life Sciences
UOM: 1 * 30 mL

New Product


Varenummer: (ENZOADI801284)
Leverandør: ENZO LIFE SCIENCES
Beskrivelse: RIPA cell lysis buffer 2, Enzo Life Sciences
UOM: 1 * 100 mL

New Product


Leverandør: G-Biosciences
Beskrivelse: These DNase- and RNase-free buffers and reagents are designed for high quality research.

Leverandør: G-Biosciences
Beskrivelse: Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.

Varenummer: (78840.)
Leverandør: Thermo Fisher Scientific
Beskrivelse: This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.
UOM: 1 * 1 Each


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