Caspase-3/7 Activity Plate Reader Assay Kit, Blue

Leverandør: STEMCELL Technologies
Ratings: Ingen bedømmelse

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100-0921
STMC100-0921EA 4556 DKK
STMC100-0921
Caspase-3/7 Activity Plate Reader Assay Kit, Blue
Assays Cellulær Assays

For detection of caspase-3/7 activity in cell lysates prepared from mammalian cells undergoing apoptosis - 200 tests.


  • Ac-DEVD-AMC detects caspase-3/7 activity
  • Excitation wavelength: 341 nm
  • Emission wavelength: 441 nm
  • Easily scalable for high-throughput assays
  • Verified for use with fluorescent plate readers


Efficiently detect caspase-3/7 activity in cell lysates prepared from mammalian cells undergoing apoptosis by using the Caspase-3/7 Activity Plate Reader Assay Kit, Blue, which includes the fluorogenic substrate Ac-DEVD-AMC and an assay buffer. This reagent kit uses a quick and simple staining protocol that is well suited for high-throughput screening and compatible with standard fluorescent plate readers.


Caspase-3 and caspase-7 have a proteolytic substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). When added to apoptotic cells, Ac-DEVD-AMC is cleaved by activated caspase-³/₇, generating a blue fluorescent signal that can be detected by standard plate readers.


Caspases are a family of proteases that play a central role in cellular apoptosis through the cleavage of select proteins, and result in the disassembly of the cell (Thornberry & Lazebnik Science, 1998). This process is switched on by pro-apoptotic signals from death receptors or stimuli such as cytotoxic reagents, which activate the initiator caspases and lead to activation of the effector caspases, caspase-3 and caspase-7 (Thornberry & Lazebnik Science, 1998). Caspase assays are useful for quantifying caspase-3/7 activity in apoptotic cells and for screening caspase-3/7 inhibitors.


CAS No: 67-68-5


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