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Leverandør: Tonbo Biosciences

Beskrivelse: The H57-597 antibody is specific for the beta chain of the mouse T cell Receptor (TCR). This cell surface protein combines with a second protein chain (alpha chain) to form the alpha-beta TCR that is expressed by NK1.1+ thymocytes, NKT cells, and the majority of peripheral T cells. A small number of T cells may express an alternative heteromer of gamma/delta protein chains, known as the g/d TCR. These receptors participate in a complex with CD3, and with the co-receptors CD4 or CD8, to recognize and respond to antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death.</p><p>The H57-597 antibody is used as a phenotypic marker for T cells expressing the alpha-beta TCR. It is also widely used to cross-link surface TCR and thereby mimic TCR-mediated cell activation or induction of apoptosis. The antibody does not cross-react with cells expressing the g/d TCR.

Varenummer: (72-8078-M001)

Leverandør: Tonbo Biosciences

Beskrivelse: Sheep anti-Rabbit IgG (H&L) - Affinity Pure, HRP Conjugate

UOM: 1 * 1 mg

Tilbud

Varenummer: (72-8068-M001)

Leverandør: Tonbo Biosciences

Beskrivelse: Goat anti-Rabbit IgG (H&L) - Affinity Pure, HRP Conjugate, min x w/ bovine, goat, human, mouse or rat IgG or serum

UOM: 1 * 1 mg

Tilbud

Varenummer: (50-8036-U100)

Leverandør: Tonbo Biosciences

Beskrivelse: Goat anti-Rabbit IgG, secondary antibody, conjugated toSureLight® R-Phycoerythrin

UOM: 1 * 1 Each

Tilbud

Varenummer: (72-8085-M001)

Leverandør: Tonbo Biosciences

Beskrivelse: Donkey anti-Sheep IgG (H&L) - Affinity Pure, HRP Conjugate, min x w/human, mouse, or rabbit IgG

UOM: 1 * 1 mg

Tilbud

Varenummer: (70-8078-M002)

Leverandør: Tonbo Biosciences

Beskrivelse: Sheep anti-Rabbit IgG (H&L) - Affinity Pure

UOM: 1 * 2 mg

Tilbud

Varenummer: (72-8074-U500)

Leverandør: Tonbo Biosciences

Beskrivelse: Goat anti-Rabbit IgG (H&L), F(ab')2 Fragment - Affinity Pure, HRP Conjugate, min x w/ bovine, human, or mouse IgG or serum proteins

UOM: 1 * 0,5 mg

Tilbud

Leverandør: Tonbo Biosciences

Beskrivelse: The PK136 antibody is specific for mouse NK1.1, a type II transmembrane lectin-like receptor and member of the killer cell lectin-like receptor (KLR) family. NK1.1 is prominently expressed on natural killer (NK) cells, and is correlated with NK cytotoxic effects toward virus-infected cells and tumor cells. NK1.1 is expressed on subsets of NKT cells in certain mouse strains (C57BL/6, FVB/N, and NZB), yet absent from others (AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129). Putative subsets of NK cells and their expression of NK1.1 antigen are of continuing interest, including NK1.1+/CD117+ (c-Kit) cells reported to be immunosuppressive for CD8+ T cells in a mechanism involving PD-1 and PD-L1 (Ehlers et al. 2012. Endocrinology. 10: 1247). The PK136 antibody may be used for detection of NK1.1 expression on mouse strains including CE, B6, NZB, C58, Ma/My, ST, SJL, and FVB. The antibody is reported to react with an epitope common to NKR-P1B and NKR-P1C alloantigenic forms of NK1.1 (Carlyle et al. 2006. J. Immunol. 176: 7511-7524).

Leverandør: Tonbo Biosciences

Beskrivelse: The MEL-14 antibody is specific for mouse CD62L, also known as L-Selectin, a cell adhesion molecule which facilitates lymphocyte "rolling" on activated vascular endothelium and homing to high endothelial venules (HEV) as immune cells transmigrate from blood into peripheral tissues. L-Selectin is a member of a family of Selectin molecules which act together with the integrin family of adhesion molecules to mediate leukocyte-endothelial interactions. L-Selectin is characteristically expressed by neutrophils, and is also found on B cells, monocytes, granulocytes, and at varying levels on naive, effector and memory T cells. It is rapidly shed upon cell activation, releasing into the circulation a soluble form whose biological role is of particular interest in cancer biology research. The MEL-14 antibody may be used as a phenotypic marker for CD62L expression on a variety of immune cell types. Please note that CD62L (L-Selectin) itself is also referred to as MEL-14 in the literature.

Leverandør: Tonbo Biosciences

Beskrivelse: This nucleic acid dye can be used to exclude non viable cells from the analysis of flow cytometry data.

MSDS

Leverandør: Tonbo Biosciences

Beskrivelse: The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.

Varenummer: (TNB-6600-KIT)

Leverandør: Tonbo Biosciences

Beskrivelse: The fragmentation of genomic DNA by cellular nucleases during the later stages of apoptosis is also one of the most easily measured features of apoptotic cells. Nuclease activity generates DNA fragments ranging from ~300 bp to 50 bp in length, resulting in a typical DNA ‘laddering’ appearance when analyzed by agarose gel electrophoresis. These fragments have exposed 3’-hydroxyl (OH) ends which can be labeled with bromolated deoxyuridine triphosphates (Br-dUTP). An enzyme, terminal deoxynucleotidyl transferase (TdT), is used to catalyze the template-independent addition of Br-dUTP to the 3’-OH ends of double or single stranded DNA. This method is often called TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) or end labeling. Sites where the Br-dUTP is incorporated can then be detected with an antibody specific to BrdU. With the APO-BrdUTM Kit cells are first labeled with Br-dUTP, and then sites of incorporation are detected through staining with a FITC anti-BrdU antibody. Samples can then be analyzed via flow cytometry. Samples that are apoptotic will stain brightly with the anti-BrdU antibody due to the substantial number of exposed 3’-OH sites, while cells that are non-apoptotic will not have incorporated significant amounts of Br-dUTP and will stain dimly. The APO-BrdU Kit is shipped in one container and consists of two packages. Upon arrival one should be stored at 2-8°C and the other at -20°C.

UOM: 1 * 1 Each

Tilbud

Leverandør: Tonbo Biosciences

Beskrivelse: The Mouse Basic T Lymphocyte backbone panel is a six-color combination of anti-mouse antibodies. This panel can serve as a backbone for identifying various T lymphocyte subsets, including naive and memory T cell populations. This panel's configuration leaves room for GFP or additional drop-in markers, if desired. Violet laser (405 nm) required for detection of CD3e violetFluorTM 450

Leverandør: Tonbo Biosciences

Beskrivelse: The RPA-2.10 antibody reacts with human CD2, an approximately 50 kDa glycoprotein, and a member of the Ig superfamily. CD2, also known as LFA-2, is a receptor for CD58 in the human and is expressed on the cell surface of 80-90% of human peripheral blood lymphocytes, a subset of NK cells, and all mature T cells. CD2 mediates lymphocyte adhesion and is involved in T cell activation.

Varenummer: (TNB-4700-L100)

Leverandør: Tonbo Biosciences

Beskrivelse: Lymphocyte Separation Medium (LSM) is a sterile-filtered iso-osmotic solution designed for the in vitro isolation of lymphocytes from diluted whole blood.

UOM: 1 * 100 mL

Tilbud

Leverandør: Tonbo Biosciences

Beskrivelse: The RPA-2.10 antibody reacts with human CD2, an approximately 50 kDa glycoprotein, and a member of the Ig superfamily. CD2, also known as LFA-2, is a receptor for CD58 in the human and is expressed on the cell surface of 80-90% of human peripheral blood lymphocytes, a subset of NK cells, and all mature T cells. CD2 mediates lymphocyte adhesion and is involved in T cell activation.