Print ...

Du Søgte efter: Kloning+buffere


25.451  results were found

Findel resultater Sorter efter
SearchResultCount:"25451"

Sort Results

Listevisning Easy View (new)

Tilfreds med din søgning? - rate den her.

Image Unavailable-70573-3

Ligation premix, Clonables™

Leverandør: Merck Millipore (Novagen)
Beskrivelse: Clonables™ 2X ligation premix is a single solution containing optimised concentrations of T4 DNA ligase, buffer, stabiliser, and cofactors needed for efficient ligation of any type of compatible DNA ends.

Product Image-AGLS200550

Random mutagenesis kits geneMorph II

Leverandør: AGILENT
Beskrivelse: The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.

Image Unavailable-AGLSG5900B

QuikChange HT protein engineering system

Leverandør: AGILENT
Beskrivelse: Protein directed evolution in just one step.

Image Unavailable-AGLS600191

Pfu DNA ligase

Varenummer: (AGLS600191)
Leverandør: AGILENT
Beskrivelse: <i>Pfu</i> DNA ligase is a recombinant enzyme derived from <i>Pyrococcus furiosus</i> hyperthermophilic marine archaebacterium that catalyses linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.
UOM: 1 * 1 Items
Image Unavailable-AGLS240218

PCR cloning kits, StrataClone

Leverandør: AGILENT
Beskrivelse: The StrataClone PCR cloning kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.

Image Unavailable-AGLS200207

Packaging extracts, Gigapack III

Leverandør: AGILENT
Beskrivelse: For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. Each packaging extract is restriction minus to optimize packaging efficiency and library representation.

Product Image-K1232

PCR cloning kit, CloneJET

Leverandør: Thermo Fisher Scientific
Beskrivelse: The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host <i>E. coli</i> cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.

Product Image-AGLS200519

Mutagenesis kits QuikChange

Leverandør: AGILENT
Beskrivelse: The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 to 14 kb) or otherwise difficult-to mutagenise plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.

Product Image-EL0016

T4 DNA ligase

Varenummer: (EL0016)
Leverandør: Thermo Fisher Scientific
Beskrivelse: This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
UOM: 1 * 1 Each

Certifikater


Product Image-27-0361-01

T4 DNA ligase

Varenummer: (27-0361-01)
Leverandør: Cytiva
Beskrivelse: T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.
UOM: 1 * 1 Each
Product Image-GA1100-10

DNA cloning kits, Gibson Assembly® HiFi 1 step kit

Leverandør: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® HiFi 1 Step Kit provides a simple and effective method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method allows researchers to achieve complex assembly of large DNA constructs, with multiple inserts, in 1 hour.

MSDS

Product Image-SYGEGA1100-4XS

Gibson Assembly® HiFi HC Cloning Kits and Master Mixes

Leverandør: SGI - DNA Synthetic Genomics
Beskrivelse: Gibson Assembly® HiFi HC 1-Step Kits and Master Mixes allow restriction digest-free, cloning of one or more DNA fragments into virtually any location of any plasmid vector in a single round of cloning.

Product Image-GA1200-10MM

Gibson Assembly® Ultra, Master mixes

Leverandør: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® Ultra reaction occurs in two steps using the addition of two master mixes in two sequential steps. In the first step, the GA Ultra Master Mix A (2X) creates single-strand DNA 5’ overhangs by chewing back DNA from the 3’ end. This reaction is cooled under conditions that allow for annealing of complementary overlap regions. Next, the Gibson Assembly® Ultra Master Mix B (2X) is added. During this step, nucleotides are incorporated into the construct to fill in the gaps in the annealed DNA fragments and nicks are sealed to create a contiguous DNA construct.

MSDS

Product Image-K1261

LIC cloning and expression kits, aLICator™, Fermentas

Leverandør: Thermo Fisher Scientific
Beskrivelse: The aLICator™ LIC Cloning and Expression System is designed for fast and efficient ligation independent cloning and tight regulation of gene expression in <i>E. coli</i>. The plate bacterial expression vectors are designed for high levels of target protein expression in concert with minimal basal (uninduced) expression, which permits expression of proteins that are toxic to <i>E. coli</i> cells.

Product Image-GA1200-10

Gibson Assembly® Ultra kit

Leverandør: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method is designed for complex assembly of 2 to 15 large DNA constructs using only small amounts (nanograms) of DNA.

MSDS

Product Image-GA1100-10MM

Gibson Assembly® HiFi 1 Step, Master mixes

Leverandør: SGI - DNA Synthetic Genomics
Beskrivelse: The Gibson Assembly® Hi-Fi 1-Step Master Mix (2X) contains a proprietary mixture of enzymes and reagents optimised to facilitate one-step assembly of double standed DNA fragments. This master mix includes a proof-reading polymerase that mediates junction repair resulting assembled constructs with low rates of junction errors and high sequence fidelity.

MSDS

Ring og spørg om pris
Lager for denne post er begrænset, men kan være til rådighed på et lager tæt på dig. Sørg for, at du er logget ind på webstedet, så tilgængelige bestand kan vises. Hvis call vises stadig, og du har brug for hjælp, bedes du ringe til os på - 43 86 87 88.
Lager for denne post er begrænset, men kan være til rådighed på et lager tæt på dig. Sørg for, at du er logget ind på webstedet, så tilgængelige bestand kan vises. Hvis call vises stadig, og du har brug for hjælp, bedes du ringe til os på - 43 86 87 88.
Dette produkt er mærket som begrænset og kan kun købes af godkendte Shipping konti. Hvis du har brug for yderligere hjælp, e-mail VWR Regulatory Department på Regulatory_Affairs@vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
Dette produkt er blevet blokeret for salg via hjemmesiden. Kontakt kundeservice for mere information.
Det originale produkt er ikke længere tilgængelig. Den viste erstatning er tilgængelig.
Produkt (er) markeret med dette symbol er udgået - sælges indtil lageret er tomt. Alternativer kan være tilgængelige ved at søge med VWR-katalognummeret ovenfor. Hvis du har brug for yderligere hjælp, bedes du kontakte kundeservice på telefon 4386 8788
1 - 16 of 25.451
no targeter for Bottom