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Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.


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Beskrivelse: Imject™ Blue Carrier™ protein is a highly soluble, mollusc-derived hemocyanin that enable simple preparation of effective immunogens with amine- or carboxyl-peptide antigens.
Varenummer: PIER77130
UOM: 1 * 100 mg
Leverandør: Thermo Fisher Scientific


Beskrivelse: Imject® Maleimide-activated mcKLH and PEGylated mcKLH enable conjugation of sulfhydryl-containing peptide haptens to elicit an immune response and antibody production against the hapten.
Varenummer: PIER77610
UOM: 1 * 1 Each
Leverandør: Thermo Fisher Scientific


Beskrivelse: Pierce™ immobilised TPCK trypsin is a serine endoprotease applicable to amino acid analysis and protein sequencing, mapping and structural studies. The immobilised form allows sample separation after treatment.
Varenummer: PIER20230
UOM: 1 * 2 mL
Leverandør: Thermo Fisher Scientific


Beskrivelse: The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.
Varenummer: 786-232
UOM: 1 * 1 Each
Leverandør: G-Biosciences


Beskrivelse: Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.
Varenummer: 786-792
UOM: 1 * 1 Items
Leverandør: G-Biosciences


Beskrivelse: Surfact-Amps™ Detergent sampler is a collection of various Surfact-Amps™ detergent solutions and selected other useful detergents for protein research methods.
Varenummer: PIER28340
UOM: 1 * 1 Each
Leverandør: Thermo Fisher Scientific


Beskrivelse: Pierce™ Trypsin Protease, MS Grade, is highly purified porcine trypsin that has been chemically modified for maximum activity and stability in proteomic applications.
Varenummer: PIER90058
UOM: 1 * 1 Each
Leverandør: Thermo Fisher Scientific

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Beskrivelse: The Mitochondria isolation Kit for cultured cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples in about 40 minutes.
Varenummer: PIER89874
UOM: 1 * 1 Each
Leverandør: Thermo Fisher Scientific


Beskrivelse: Organelle enrichment kit for lysosomes enables enrichment of intact organelles from cells and tissue. The kit provides the media and reagents necessary to accomplish subcellular fractionation using high-speed density gradient centrifugation.
Varenummer: PIER89839
UOM: 1 * 1 Each
Leverandør: Thermo Fisher Scientific


Beskrivelse: BugBuster® master mix combines BugBuster® protein extraction reagent with Benzonase® Nuclease and rLysozyme™ solution in one convenient reagent. It allows for maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The master mix provides sufficient reagents for protein extraction from 20 g and 100 g cell paste.
Varenummer: 71456-3
UOM: 1 * 100 mL
Leverandør: Merck Millipore (Novagen)


Beskrivelse: The CytoBuster™ protein extraction reagent is a proprietary formulation of detergents optimised for efficient extraction of soluble proteins from mammalian and insect cells. The gentle, non-ionic composition of the reagent enables isolation of functionally active endogenous or expressed proteins without a need for secondary treatment such as sonication or freeze/thaw.
Varenummer: 71009-4
UOM: 1 * 250 mL
Leverandør: Merck Millipore (Novagen)


Beskrivelse: Native endoproteinase Asp-N from <i>Pseudomonas fragi.</i> Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
Varenummer: 324708-2
UOM: 1 * 1 Each
Leverandør: Merck Millipore (Calbiochem‎)


Beskrivelse: ProteoExtract® Kits cover the different steps of the proteomics workflow, from protein extraction and abundant protein removal to concentration of protein mixtures and removal of interfering substances, digestion of proteins and selective capturing of phosphopeptides. These kits work in concert with one another to provide protein samples that are directly compatible with downstream analytical applications.
Varenummer: 539790-1
UOM: 1 * 1 Each
Leverandør: Merck Millipore (Calbiochem‎)


Beskrivelse: BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for protein extraction from 20 g or 100 g cell paste.
Varenummer: 71370-3
UOM: 1 * 1 Items
Leverandør: Merck Millipore (Novagen)


Beskrivelse: The Column-PROTEIN-Concentrate™ Kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilises any protein in a low salt buffer between pH 2 to 12. The binding capacity is ~0,5 mg protein/ml Protein Binding Resin.
Varenummer: 786-126
UOM: 1 * 1 Each
Leverandør: G-Biosciences

MSDS


Beskrivelse: Native endoproteinase Lys-C from <i>Lysobacter enzymogenes</i>. Serine protease that specifically hydrolyses amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease: protein by weight) for sequence analysis.
Varenummer: 324715-5
UOM: 1 * 1 Each
Leverandør: Merck Millipore (Calbiochem‎)


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