PIPES free acid, white powder

Leverandør: MP Biomedicals
Ratings: Ingen bedømmelse

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0219025710 0219025791 0219025780 0219025790
ICNA0219025710EA 264 DKK
ICNA0219025710 ICNA0219025791 ICNA0219025780 ICNA0219025790
PIPES free acid, white powder
PIPES

PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer. Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the sesquisodium salt and the disodium salt or dipotassium salt, titrating to the appropriate pH.


Zwitterionic Buffer, Effective buffering range: 6,1 - 7,5 (Merck Index reports 6,0 to 8,5 ), ΔpK/ΔT: 0,0085, pKa: 6,8 at 25 °C ; 6,66 at 37 °C


PIPES is used to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue tissue samples. This will help prevent the loss of lipids. It is used to buffer glutaraldehyde and formaldehyde when fixing fungal zoospores for fluorescence microscopy and electron microscopy.


PIPES free acid is not very water soluble (approximately 1 g/l at 100 °C) but will solubilise in 0,5 M NaOH; PIPES salts are water soluble (usually resulting in clear, colourless solutions).

Formel: C₈H₁₈N₂O₆S₂
Kogepunkt: 499 °C (1013 hPa)
Smeltepunkt: 300 °C
Opbevaringstemperatur: Stue temperatur
MDL Number: MFCD00006159
CAS-nummer: 5625-37-6
EINECS: 227-057-6
Merck Index: 12,07633
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Specifikationer Testresultater

Identity Test Passes
FTIR Conforms to Standard
UV/Vis: OD260 (0,1 M in 0,2 M NaOH) ≤0,05
UV/Vis: OD280 (0,1 M in 0,2 M NaOH) ≤0,05


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