Corning® Nu-Serum™ Serum Replacements, Corning
Leverandør: CorningSamlet vurdering
Gennemsntilig vurdering
Corning® Nu-Serum™ vækstmedieadditiv giver et lavproteinalternativ til nyfødt kalv, føtalt bovint serum og andre sera, der rutinemæssigt anvendes til cellekulturer. Det lave indhold af proteiner fremmer oprensningen af proteiner, virusproduktionen, produktion og screening af monoklonale antistoffer og øger frekvensen med hensyn til vellykket transfektion af celler.
Formulation: Frozen solution containing 25% newborn serum, EGF, ECGS, insulin, human transferrin, triiodothyronine, progesterone, estradiol-17ß, testosterone, hydrocortisone, selenous acid, o-phosphorylethanolamine, glucose, amino acids, vitamins, and other trace elements and nutrients contained in the Ham's F12 medium base.
Tested at a concentration of 10% for the ability to stimulate a >= 100-fold increase in growth of BHK-21 and a >= 13-fold increase in growth of BALB/c-3T3 cells. Tested for total protein content, pH, and osmolarity (information included with product). Filtered (0,2 µm membrane) and tested and found negative for the presence of bacteria, fungi, mycoplasma, and viruses (bovine diarrhea virus, bovine parvovirus, bovine adenovirus, reovirus, and rabies virus); also tested for the absence of cytopathic effects (CPE), inclusion bodies and hemadsorption. Tested for endotoxin (LAL assay).
Monoclonal antibody production and screening and increases the frequency of successful transfection of cells.
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