Du Søgte efter: Nucleic Acid Purification Kits and Reagents

Leverandør: OMEGA BIO-TEK

Beskrivelse: Oprensning af DNA fra gelfragmenter er en almindelig teknik til isolering af et specifkt DNA fragment. De fleste metoder fjerner ikke al agarosen eller ødelægger DNAet eller giver lavt udbytte.

MSDS Certifikater

Leverandør: OMEGA BIO-TEK

Beskrivelse: The E.Z.N.A.® bacterial DNA kit allows the rapid and reliable isolation of high quality total cellular DNA from a wide variety of bacterial species. This kit uses optimised lysis condition and up to 1×10⁹ bacterial cells can be processed for each column. There are no organic extractions, thus reducing plastic waste and hands on time to allow multiple samples to be processed in parallel. Bacterial cells are grown to log-phase and harvested. The cell wall is removed by lysozyme digestion and bead beating, followed by protease digestion. Following lysis, binding conditions are adjusted and the sample is applied to a HiBind DNA spin-column. Two rapid wash steps remove trace salts and protein contaminants, and DNA is finally eluted in water or elution buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

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Leverandør: OMEGA BIO-TEK

Beskrivelse: Isolate DNA from plant or fungal samples using spin columns.

MSDS

Varenummer: (R6830-02)

Leverandør: OMEGA BIO-TEK

Beskrivelse: RNA-Solv® Reagent is a one reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and guanidine isothiocyanate, is a modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenised and lysed in RNA-Solv® Reagent, which maintains the integrity of the RNA while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.

UOM: 1 * 200 mL

MSDS Certifikater Tilbud

Leverandør: Merck

Beskrivelse: The device utilises gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.

Leverandør: OMEGA BIO-TEK

Beskrivelse: Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid kits integrate an efficient endotoxin removal step into the plasmid purification procedure to produce high quality transfection grade plasmid.

MSDS Certifikater

Leverandør: OMEGA BIO-TEK

Beskrivelse: The E.Z.N.A.® Blood RNA Kit is designed for the isolation of total intracellular RNA from up to 1 ml of fresh, or frozen whole blood treated with any common anticoagulant such as heparin, EDTA or acid-citrate-dextrose. The procedure completely removes contaminants and enzyme inhibitors making total RNA isolation fast, convenient and reliable. Red blood cells are selectively lysed and white cells are collected by centrifugation. After lysis of white blood cells under denaturing conditions that inactivate RNases, the lysate is homogenised with a homogeniser spin column. The sample is then applied to a HiBind® spin column. Cellular debris and other contaminants such as haemoglobin are effectively washed away and high-quality RNA is finally eluted in DEPC treated water.

MSDS Certifikater

Leverandør: OMEGA BIO-TEK

Beskrivelse: E.Z.N.A.SP Plant DNA Kit er specielt designet til hurtig og pålidelig isolering af DNA fra en mængde forskelligt plantemateriale. Op til 100mg frisk eller 30mg tørret prøve can oprenses på under 40 minutter. Oprenset DNA er velegnet til de fleste downstream applikationer, såsom PCR, restriktionsskæringer og hybridiseringer.

MSDS

Leverandør: Corning

Beskrivelse: The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilises a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimised buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

Leverandør: Corning

Beskrivelse: Removing excess dye terminator is an essential step prior to Sanger sequencing. Carryover of the excess dye into the sequencing reactions may result in dye blobs resulting in inaccurate results. The AxyPrep™ Mag DyeClean kit utilises a paramagnetic bead-based purification system for Sanger sequencing reaction dye terminator clean-up. The protocol is simple and comprises binding, washing and elution steps which can be performed directly in the thermal cycling plate. This kit requires no centrifugation or filtration steps.

Varenummer: (MAG-PCR-NM-5)

Leverandør: Corning

Beskrivelse: The AxyPrep™ Mag PCR Normalizer Kit utilizes a paramagnetic bead-based purification system for PCR clean up.

UOM: 1 * 1 Each

Tilbud

Leverandør: OMEGA BIO-TEK

Beskrivelse: Isolate host genomic, bacterial, fungal spore, and viral DNA and viral RNA from tissue, urine, serum, and fecal samples using magnetic beads.

Leverandør: Quantabio

Beskrivelse: Rapid extraction and purification of high-quality total RNA from cultured cells or tissue.

Leverandør: OMEGA BIO-TEK

Beskrivelse: The E.Z.N.A.® Forensic DNA Kit is designed to provide a rapid and easy method for the isolation of genomic DNA from forensic samples such as dry blood, buccal swabs, and semen samples for consistent PCR and Southern analysis. This kit can also be used for the preparation of genomic DNA from mouse tail snips, whole blood, buffy coat, serum, and plasma.

MSDS

Varenummer: (D5032-03)

Leverandør: OMEGA BIO-TEK

Beskrivelse: The E.Z.N.A.® SQ blood DNA kit provides a reliable method for the isolation of high molecular weight genomic DNA from fresh, frozen, or anti-coagulated whole blood. The method can also be used for preparation of genomic DNA from buffy coat, bone marrow or cultured cells. There is no need for phenol/chloroform extractions, and time consuming steps such as CsCl gradient ultracentrifugation are eliminated. DNA purified using the E.Z.N.A.® SQ blood DNA method is ready for downstream applications such as PCR, southern blotting and restriction enzyme digestion.

UOM: 1 * 300 mL

Certifikater Tilbud

Leverandør: OMEGA BIO-TEK

Beskrivelse: The E.Z.N.A.® Bacterial RNA kit is designed for isolation of high-quality total RNA from variety of bacterial strains. Up to 1×10⁹ log-phase bacterial cells can be processed. This kit uses an improved lysis procedure to ensure the complete lysis of bacterial cells. Purified RNA is suitable for downstream applications such as RT-PCR and hybridisation techniques.

MSDS Certifikater