Beskrivelse: The Pearl™ Monoclonal IgG Purification Kit allows for the rapid purification of antibodies from cell culture supernatant and ascites fluid. The Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulphate precipitation.

Varenummer: 786-802

UOM: 1 * 1 Each

Leverandør: G-Biosciences

Tilbud

Beskrivelse: The rPSL1a is an Escherichia coli recombinant of carbohydrate-binding protein (Lectin) derived from Polyporus squamosus. This lectin preferably binds to a [Siaα(2-6)Gal] glycan epitope (It very slightly interacts with lactose.).
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This rPSL1a-LecBeads (1 ml gel) shows a binding capacity of about 10 mg or more human Transferrin [hTF] through its binding to a terminal [Siaα(2-6)Gal] glycan epitope on hTF.

Varenummer: TCIAR0235-1VIAL

UOM: 1 * 1 Each

Leverandør: TCI

Tilbud

Beskrivelse: The Pearl™ Antibody Clean Up Kit is designed for the rapid clean up of antibody solutions using a combination of our Pearl™ IgG Purification Resin to remove the protein stabilisers and SpinOUT™ desalting columns to ensure the antibody solutions are in an optimal buffer for clean up. It binds high abundant, non IgG proteins (i.e. BSA and gelatin) and allows the IgG molecules to pass through in a physiological buffer. For the purification of ten 0,5 ml IgG samples with up to 1% BSA and gelatin.

Varenummer: 786-803

UOM: 1 * 1 Each

Leverandør: G-Biosciences

Tilbud

Beskrivelse: Concanavalin A (Con A) agarose consists of Con A coupled to 6% agarose by the cyanogen bromide method. Con A is a tetrameric metalloprotein lectin isolated from <i>Canavalia ensiformis</i> (jack bean). It is used for the purification of glycoproteins, polysaccharides and glycolipids as it binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl and sterically related residues. It has also be used in other application areas including purification of enzyme-antibody conjugates, purification of IgM and separation of membrane vesicles. As stated above, it is a metalloprotein and to maintain its binding characteristics the presence of both Mn²⁺ and Ca²⁺ is essential. Each subunit of Con A utilises one calcium and one manganese ion and these cations can be removed under acidic conditions abolishing the carbohydrate-binding activity.

Varenummer: 786-218

UOM: 1 * 100 mL

Leverandør: G-Biosciences

Tilbud

Beskrivelse: Anti-His affinity resins are designed for purification of His-tagged protein. Composed of agarose and conjugated THE™ anti-His monoclonal antibody, they are compatible with most commonly used reagents at certain concentrations.

Varenummer: GENSL00439-5

UOM: 1 * 5 mL

Leverandør: Genscript

Tilbud

Beskrivelse: ImmunoReagents Goat IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Goat IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Varenummer: IMMRIR1010

UOM: 1 * 2 mL

Leverandør: ImmunoReagents

Tilbud

Beskrivelse: Cobalt chelating resin, 1 ml columns, for the purification of 6X His proteins

Varenummer: 786-454

UOM: 1 * 25 Items

Leverandør: G-Biosciences

Tilbud

Beskrivelse: This avidin resin is designed for the single step small and large scale affinity purification of proteins and antibodies with a biotin tag. The resin can also be used for immunoprecipitations using biotin labelled antibodies.

Varenummer: 786-593

UOM: 1 * 5 mL

Leverandør: G-Biosciences

Tilbud

Beskrivelse: Glutathione resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 4% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of ~8 mg GST/ml resin. Supplied as slurry in 20% ethanol.

Varenummer: 786-312

UOM: 1 * 500 mL

Leverandør: G-Biosciences

Tilbud

Beskrivelse: Thiophilic adsorption or thiophilic chromatography is a routinely used technique for the low cost, simple purification of immunoglobulins. Thiophilic adsorption was first developed by Porath et al in 1984 and is a group specific, salt-dependent purification technique that has distinct affinity towards immunoglobulins and α2- macroglobulins. The thiophilic adsorption works on the principle that some proteins in high salt are able to bind to an immobilised ligand that contains a sulfone group in proximity to a thioether group. The bound proteins are then eluted in decreasing salt concentrations. The thiophilic resin binds immunoglobulins, including IgG, IgY and IgM, from serum, ascites or tissue culture supernatants and the purified immunoglobulins are then eluted in a near neutral aqueous buffer.

Varenummer: 786-266

UOM: 1 * 1 Each

Leverandør: G-Biosciences

Tilbud

Beskrivelse: ImmunoReagents Mouse IgG Affinity Prep resins are prepared using highly purified species specific immunoglobulins. This resin is suitable for removal of proteins recognising Mouse IgG from liquid solutions, including traditional Immunoabsorptions and Immunoprecipitation protocols.

Varenummer: IMMRIR1013

UOM: 1 * 2 mL

Leverandør: ImmunoReagents

Tilbud

Beskrivelse: The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin format columns for the desalting and buffer exchange of protein solutions ranging from 5 µl through to 4 ml sample volumes. The SpinOUT™ columns are available in three MWCO sizes for >700, >6000 or >30 000 dalton proteins and are suitable for samples containing as little as 20 µg protein/ml.

Varenummer: GENO786-897

UOM: 1 * 50 Items

Leverandør: G-Biosciences

Tilbud

Beskrivelse: Starter reagent kit for multiple affinity removal LC columns.

Varenummer: AGLS5185-5991

UOM: 1 * 25 Items

Leverandør: AGILENT

Tilbud

Beskrivelse: Protein A Sepharose™ is a suspension of beads conjugated to native Protein A derived from <i>Staphylococcus aureaus</i>. Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species, including human, mouse, rat IgG2c, cow IgG2c, goat IgG2, sheep IgG2 and rabbit.

Varenummer: ROCKPA50-00-0005

UOM: 1 * 5 mL

Leverandør: Rockland Immunochemicals

Tilbud

Beskrivelse: Following digestion of proteins with proteases, the addition of Pep-Extract™ rapidly elutes digested peptides in under 20 minutes. The eluted peptides can be used directly in mass spectrometry analysis without any additional clean up. Contains sufficient for 500 protein spots.

Varenummer: 786-243

UOM: 1 * 1 Each

Leverandør: G-Biosciences

Tilbud

Beskrivelse: The Pearl™ IgG purification kits are designed for the one step purification of IgG antibodies from serum. The supplied Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The purified IgG molecules can be stored or used in further downstream applications without further clean-up, such as ammonium sulphate precipitation.

Varenummer: 786-798

UOM: 1 * 1 Each

Leverandør: G-Biosciences

Tilbud